Complex medium supplements optimized for the reduction of animal-derived components in vaccine production media

Introduction A series of cell-line specific complex media supplements have been developed for enhancing performance of various vaccine production systems. Created using in-house media optimization methods, these supplements are manufactured using innovative process technology which allows for the formulation of complex and/or chemically defined animal-component free media additives into a single homogenized functional supplement. These supplements have been optimized for individual cell lines, and have proven to be suitable for use in a range of basal media. Data are presented which demonstrate the effectiveness of these optimized supplements for application as performance enhancers, and as a vehicle for serum-reduction in CEF, MDCK and BHK culture media. While these particular supplements do contain some undefined components, preliminary research indicates that this same technology may be applied to chemically-defined, multi-component supplements.